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Medicine Science

Artificial Sweeteners Are Toxic To Digestive Gut Bacteria, Study Finds (cnbc.com) 192

According to a study published in the journal Molecules, researchers found that six common artificial sweeteners approved by the FDA and 10 sport supplements that contained them were found to be toxic to the digestive gut microbes of mice. CNBC reports: Researchers from Ben-Gurion University of the Negev in Israel and Nanyang Technological University in Singapore tested the toxicity of aspartame, sucralose, saccharine, neotame, advantame, and acesulfame potassium-k. They observed that when exposed to only 1 milligram per milliliter of the artificial sweeteners, the bacteria found in the digestive system became toxic. According to the study, the gut microbial system "plays a key role in human metabolism," and artificial sweeteners can "affect host health, such as inducing glucose intolerance." Additionally, some of the effects of the new FDA-approved sweeteners, such as neotame, are still unknown.

However, the study found that mice treated with the artificial sweetener neotame had different metabolic patterns than those not treated, and several important genes found in the human gut had decreased. Additionally, concentrations of several fatty acids, lipids and cholesterol were higher in mice treated with neotame than in those not. Because of the widespread use of artificial sweeteners in drinks and foods, many people consume them without knowing it.

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Artificial Sweeteners Are Toxic To Digestive Gut Bacteria, Study Finds

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  • by Anonymous Coward

    Makes me sick

  • One milligram of sweetener per milliliter of sweetener? What?

    • Re: (Score:3, Informative)

      by wierd_w ( 1375923 )

      AC, this is not difficult.

      The substances being tested are crystalline solids at room temperature. They are dissolved in a solvent.

      In this case, at a concentration of 1mg : 1mL, as an aqueous solution.

      Granted, that is pretty damn concentrated. But still.

      • Re: (Score:3, Informative)

        by hrafn42 ( 227947 )

        Granted, that is pretty damn concentrated.

        Particularly for sucralose, which is 600x as sweet as sugar. 1mg/ml would be sickly sweet. I generally use 0.1mg/ml in making my own sugar-free cordials (I'm diabetic).

      • It's like saying beer contains 8% beer by volume.

        • Re: (Score:2, Troll)

          by wierd_w ( 1375923 )

          That would be true if beer was a crystalline solid at room temperature.

          Which it is not.

          • It wouldn't be true in any case, because the solute is one thing and when you mix it with a solvent it becomes another thing. There's a word for that - a solution.

            It's like using the same word in the same sentence to mean both salt and brine.

          • by Zorro ( 15797 )

            Except Siberia.

      • by Nutria ( 679911 )

        In this case, at a concentration of 1mg : 1mL, as an aqueous solution.

        Granted, that is pretty damn concentrated. But still.

        That scales to 1g/L, doesn't it? To me, that doesn't seem anywhere near as concentrated as the absurdly high dosages used in cancer tests.

        • by dfghjk ( 711126 )

          Absurdity is not defined by whether something is more or less absurd than something else that is absurd.

          1 mg/ml is vastly higher than would actually be used. Furthermore, it was the minimum threshold for a positive response for only one of the tested sweeteners. All other required higher concentrations still, including neotame which is at least 10x stronger than Sucralose (the one with a response at 1mg/ml). With neotame, the concentrations required would be 100 - 1000 times stronger than what would be t

  • by fredrikv ( 521394 ) on Friday October 05, 2018 @03:14AM (#57429548)

    Please correct the text, the referenced study is about neotame only and does not investigate the other mentioned molecules or products. The CNBC article is probably to blame as it is misleading on which results were obtained in which study.

    • by mentil ( 1748130 ) on Friday October 05, 2018 @03:55AM (#57429646)

      I skimmed through that study. It found that neotame disrupted the balance of the microbiome, metabolism of certain vitamins and other nutrients, and the metabolic pathways. However, body weight of the experimental and control groups were the same after 4 weeks, which was buried in the end although it'd been predicted that body weight should've dropped. I'm unsure if the disruption is enough to potentially lead to malnutrition, although metabolic syndrome and various gut disorders are specifically mentioned in the paper as possible effects.
      Also, apparently it's considered humane to euthanize mice with CO2? I'd think that'd cause painful asphyxiation.

      • by dargaud ( 518470 )

        Also, apparently it's considered humane to euthanize mice with CO2? I'd think that'd cause painful asphyxiation.

        It's painless: you just pass out.

        • by jtgd ( 807477 ) on Friday October 05, 2018 @05:24AM (#57429862)
          No, you're thinking of nitrogen. CO2 will make you die in extreme panic.
          • Or maybe CO, which is OK because it's one less.

            • by necro81 ( 917438 ) on Friday October 05, 2018 @08:20AM (#57430426) Journal

              Or maybe CO, which is OK because it's one less.

              Actually, yes, death by carbon monoxide poisoning would be preferrable to carbon dioxide.

              In the former case, you get drowsy, fall asleep, and eventually asphyxiate. CO binds to hemoglobin, preventing your blood from taking up oxygen. You die of hypoxia, which is generally thought of being pretty painless. (If hypoxia were painful, then high-altitude mountaineering would be impossible, rather than just merely hard.) A similar death would occur in a depressurized airline cabin if you don't put your mask on. CO poisoning happens to a 100s of people each year in the U.S. due to faulty heating systems. One reason deaths result is because people aren't aware that it's even happening - they just fall asleep, then die. This is why your home should have at least one CO detector per floor.

              Carbon dioxide poisoning, on the other hand, is definitely a rough way to go. Hold your breath for a while and you'll see what I mean. That panic you're feeling, the tightening of the chest, the burning, that's caused by your brainstem realizing your blood has built up too much CO2. At low levels, high CO2 concentration in the air will make your irritable, give you a headache, and generally make it hard to function at your best. (Astronaut Scott Kelly complained about this a lot during his year on the ISS.) Taken higher, and your entire body becomes acidotic, and that feeling of drowning becomes all-encompassing. Eventually, your metabolism will break down at a cellular level as your blood becomes saturated with CO2. You'll have passed out long before that, but your last conscious moments will probably be in agony.

              • Carbon dioxide poisoning, on the other hand, is definitely a rough way to go. Hold your breath for a while and you'll see what I mean.

                Slightly raised levels of CO2 are definitely uncomfortable, but that doesn't meant that close to 100% CO2 is similar.

          • Re: (Score:2, Interesting)

            by Megol ( 3135005 )

            Mice aren't humans and people actually tries to minimize suffering of the animals so this have been studied.

          • It depends on the concentration. Above 95% concentration of CO2 unconsciousness occurs in seconds. It's used as a low-stress way stun pigs before slaughter and results in better meat quality than electrocution or a bolt gun.

            The process is informative. A group of pigs (they prefer to be in groups) goes down a cute into an elevator. The hatch closes and the elevator drops into the pit. If they make any noise at all (other than falling over) after the elevator gets in the pit then something is wrong.

        • It's painless: you just pass out.
          Depends how you define "painless". You get a panic attack ... not knowing what to do and how to escape: before you pass out.

      • by Rei ( 128717 ) on Friday October 05, 2018 @06:59AM (#57430122) Homepage

        The study was published in a MDPI journal. MDPI has some serious reputational problems [wikipedia.org] (more here [universityaffairs.ca]).

        The concentrations given were around a thousand of times a normal neotame dose (equivalent to dozens of milligrams for an adult human, where normal daily neotame intake is in the dozens of micrograms [europa.eu]) (or over a hundred times a normal dose if you accept their 12,5x human:mouse exchange factor, although that seems misguided since they're testing effects on bacteria, not direct effects on the animal). One thing that's notably absent IMHO is the glaring omission of discussion of the mice's food consumption. It's not even clear whether the pelleted diet is ad libitum or whether just the water is ("standard pelleted rodent diet and tap water ad libitum"). If the pelleted diet is ad libitum then it seems utter incompetence to not discuss changes in dietary consumption when doing a gut flora study.

      • Re: (Score:2, Informative)

        by Anonymous Coward

        Also, apparently it's considered humane to euthanize mice with CO2? I'd think that'd cause painful asphyxiation.

        That's a common misconception. It actually depends on the concentration. In a pure CO2 atmosphere, the oxygen levels in the blood will drop so fast that the body will lose consciousness before the brain becomes aware of suffocation. It only gets painful if CO2 levels rise slowly enough, for example when breathing in an enclosed space.

      • by Gravis Zero ( 934156 ) on Friday October 05, 2018 @07:59AM (#57430360)

        Also, apparently it's considered humane to euthanize mice with CO2? I'd think that'd cause painful asphyxiation.

        It depends on the air mixture. Inhaling a dense cloud of CO2 results in immediate unconsciousness followed by death.

        This phenomenon has been documented as it happened to a village by a lake where tons of CO2 spewed out from a lakebed and killed many people by the lake. [history.com] The people who suffered were the ones who survived but were still exposed as it caused them to cough until they bled.

      • by hey! ( 33014 )

        Four weeks is not very long.

        Most people gain weight very slowly, over the course of years. For example I gained 100 pounds over 20 years (most of which I've subsequently lost, but that's another story); that works out to about 6 grams per day; over the course of 4 weeks that works out to about six ounces, a difference that would be extremely challenging to detect against the background noise of hydration variation.

    • by Anonymous Coward on Friday October 05, 2018 @04:40AM (#57429744)

      The CNBC article confuses two studies: Neotame in mice [mdpi.com] and Artificial sweeteners on a bacterial panel [mdpi.com].

      The second of these does indeed test the toxicity of all the named sweeteners, albeit only on a model of gut bacteria (bioluminescent E.coli) in a laboratory setting.

      Sucralose and neotame were found to inhibit E.coli bioluminescence. Saccharin, aspartame and ace-k induced it.

      What does that mean? Your guess is as good as mine.

      • Re: (Score:3, Funny)

        by Hognoxious ( 631665 )

        Wait. So how much Diet Coke do I need to quaff before my shit glows in the dark?

      • The CNBC article confuses two studies: Neotame in mice [mdpi.com] and Artificial sweeteners on a bacterial panel [mdpi.com].

        The second of these does indeed test the toxicity of all the named sweeteners, albeit only on a model of gut bacteria (bioluminescent E.coli) in a laboratory setting.

        Sucralose and neotame were found to inhibit E.coli bioluminescence. Saccharin, aspartame and ace-k induced it.

        What does that mean? Your gas is as glowy as mine.

        Ftfy

      • A few years back, there was a short and small study about common sweeteners of the time, including sugar, on actual humans, that found a notable loss of gut bacteria - One of many metrics being tested. The result was a surprise at that stage.

        • by evanh ( 627108 )

          I should have said "... that found a notable loss of gut bacteria" from only the artificial sweeteners.

          The two that didn't produce this effect was sugar and stevia.

    • by afxgrin ( 208686 ) on Friday October 05, 2018 @05:16AM (#57429830)

      Directly from the paper in the summary:

      Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
      Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
      Department of Population Health and Pathobiology, North Carolina State University, Raleigh, NC 27607, USA

      None of these are Ben-Gurion University or Nanyang Technological University.
      The correct paper is:
      Measuring Artificial Sweeteners Toxicity Using a Bioluminescent Bacterial Panel [mdpi.com]

    • There is more than 1 study, a quick click on the "artificial sweeteners" keywords at the end of the abstract, will provide the other studies.
  • They just looked at the abstract and did not even quote that correctly. The article is only about neotame and the bacteria did not become toxic, they died.
    There was no increased concentrations of several fatty acids, lipids and cholesterol in the mice, but in their feces. This is just an attempt to create panic.

    • by Sique ( 173459 )
      They were actually more diligent than you, as the second link goes to a CNN article, which quotes from a press release that links to a different article, and this article [mdpi.com] indeed was reporting about six artificial sweeteners: "In this study, the relative toxicity of six FDA-approved artificial sweeteners (aspartame, sucralose, saccharine, neotame, advantame and acesulfame potassium-k (ace-k)) and that of ten sport supplements containing these artificial sweeteners, were tested using genetically modified biol
      • by txoof ( 553270 )

        The second article is indeed better, but it is an in-vitro study. While the in-vitro results are interesting and definitely an indicator as to how these substances might act in-vivo, it is not the whole story.

        The slashdot headline and summary is a little misleading and alarmist, but the takeaway, that artificial sweeteners might not be good for our gut biome, is probably worth further investigation.

      • were tested using genetically modified bioluminescent bacteria from E. coli."

        They're toxic to E.coli? I just might be OK with that.

  • by Anonymous Coward on Friday October 05, 2018 @03:26AM (#57429570)

    To be clear here, because TFS is a mess, there are two separate papers by two separate research teams. The paper described in the first line of the summary is this one [mdpi.com], looking at a mix of supplements and how they affected bioluminescent reporting in bacteria. The paper which is linked in the first sentence ( this one [mdpi.com]) is the one which looked at Neotame exposed mice, referred to in the last paragraph of TFS.

    • by ath1901 ( 1570281 ) on Friday October 05, 2018 @04:59AM (#57429788)

      Thanks for the links! Both the summary and the cnbc article link the wrong one.

      As for the CNBC article, they say "They observed that when exposed to only 1 milligram per milliliter of the artificial sweeteners, the bacteria found in the digestive system became toxic" but is that really what the study shows?

      The bioluminescent bacteria, which luminesce when they detect toxicants, act as a sensing model representative of the complex microbial system. Both induced luminescent signals and bacterial growth were measured. Toxic effects were found when the bacteria were exposed to certain concentrations of the artificial sweeteners. In the bioluminescence activity assay, two toxicity response patterns were observed, namely, the induction and inhibition of the bioluminescent signal. An inhibition response pattern may be observed in the response of sucralose in all the tested strains: TV1061 (MLIC = 1 mg/mL), DPD2544 (MLIC = 50 mg/mL) and DPD2794 (MLIC = 100 mg/mL). It is also observed in neotame in the DPD2544 (MLIC = 2 mg/mL) strain. On the other hand, the induction response pattern may be observed in its response in saccharin in TV1061 (MLIndC = 5 mg/mL) and DPD2794 (MLIndC = 5 mg/mL) strains, aspartame in DPD2794 (MLIndC = 4 mg/mL) strain, and ace-k in DPD2794 (MLIndC = 10 mg/mL) strain

      I am not a chemist/biologist and can not decipher that but

      a) Bacteria in the gut did not become toxic. It was a genetically engineered bioluminescent strain of e-coli that signals when it detects toxicants.
      b) There are many reported numbers ranging from 1 mg/mL to 100 mg/mL as well as different responses to different sweeteners.

      So if I just assume the limit for toxicity is 1 mg/mL like they say, a can of diet coke contains 125 mg of Aspartame and is 8 oz = 240 mL which is about 0.5 mg/mL and thus not-toxic. Also, if the other fluids in the body dilute the coke it should be even more safe since it is the concentration that matters. Is that a reasonable conclusion?

      • Is it concentration? Or net dosage? Many diet soda drinkers can consume a 2-liter bottle in the course of a day quite esily.

        • The point everyone seems to miss amidst the moral panic is that the risk from consuming an artificially-sweetened 2L bottle of soda might not be ZERO, but it's still a huge net improvement over the known, documented harm likely to arise from the daily consumption of a 2L bottle of soda sweetened with sugar or HFCS.

          Daily consumption of 2L of diet soda: theoretical changes in gut bacteria.

          Daily consumption of 2L of regular soda: significantly elevated risk of diabetes and obesity.

          The worst thing that could po

      • by religionofpeas ( 4511805 ) on Friday October 05, 2018 @06:06AM (#57429978)

        The gut has many different bacteria. This study only tested e-coli.

        Many different foods and spices could influence gut bacteria. This study didn't do any controls.

        Many sweeteners are already broken down/absorbed in the small intestine, whereas e-coli only lives in the colon. This study didn't compensate for that.

        Conclusion: lazy and deceptive study. What is alarming is that fairly obscure/crap (not published in major journal) studies like this show up all over the place. I've seen it pop up several times in tweets, reddit, and now on slashdot. Makes you wonder why so many news outlets feel compelled to pick up such a poorly done study.

      • by dfghjk ( 711126 )

        If you are looking at aspartame, why use a toxicity threshold for Sucralose? Aspartame's minimum concentration was 4 ml/ml, making your argument even stronger.

  • by GeLeTo ( 527660 ) on Friday October 05, 2018 @03:57AM (#57429656)
    The summary is a mess. Still, there are quite many studies that indicate that non-nutritive sweeteners are associated with increased risk to develop obesity, metabolic syndrome, type 2 diabetes and cardiovascular disease: https://www.ncbi.nlm.nih.gov/p... [nih.gov] And their effects on gut bacteria is the main suspect for this.
    • Since diabetics, obese people, and those with related coronary disease often try to reduce weight, the association would seem to be cause and effect of those disorders causing the use of non-nutritive sweeteners. High insulin levels at the onset of Type 2 diabetes are also associated with causing increased hunger and weight gain, _before_ the weight increase of many victims of Type 2 diabetes.

      I'm not discounting all effects described in the study you mention, merely trying to point out that "association" do

  • What about Stevia?
  • It basically starts killing my gut bacteria.

    And as anyone who has taken extensive antibiotic regimens can tell you.
    Killing off your gut flora transforms you into ASS CANNON MAN!

    • by Ogive17 ( 691899 )
      So that explains the 2-3 days after I return home from visiting Mexico....
      • by Chas ( 5144 )

        No. That's the water. Too much sulfur in it.
        But, essentially, same effect. You become The Mighty Colon Blow.

  • by Tough Love ( 215404 ) on Friday October 05, 2018 @06:59AM (#57430124)

    Link to the original paper. [mdpi.com] Not paywalled, sweet. (see what I did there?)

    The summaries, mindlessly cut and pasted several levels deep including in the /. summary, really suck. According to the summaries, the bacteria "became toxic". No they didn't, please get a clue.

  • Read the paper (Score:3, Informative)

    by Karmashock ( 2415832 ) on Friday October 05, 2018 @08:34AM (#57430482)

    ... if you read the paper... they are administering 2.5 times the FDA recommended dose of Sweat and Low... the mice showed no signs of ill health or altered behavior... and the "toxic" is a little bit of a stretch... there were changes in gut bacteria populations but that happens whenever you change a diet to anything else.

    Did someone else get a different read on this thing? I went through the "method" section which is where I always start whenever I read a paper. Straight to the method... aka what did you actually do?

  • by atrex ( 4811433 ) on Friday October 05, 2018 @09:47AM (#57430774)
    Step 1) Get the country hooked on sugar - Check and Profit
    Step 2) Make a fortune of diabetes treatments - Check and Profit
    Step 3) Develop and market tons of different artificial "no/low calorie" sweeteners and market to people now suffering from obesity - Check and Profit
    Step 4) ???
    Step 5) Profit Profit Profit!
    • Carbs, fats and protein don't make you fat, you have to eat an excess. How did they force you to do that?
      • by atrex ( 4811433 )
        Quite easily actually. It's called advertising. Not to mention decades of selling fast food meals that have enough calories in them for an entire day not just one meal. Check out the history regarding the size of wine glasses over time as an example. It's very easy to psychologically trick people into consuming both the wrong foods and too much of the wrong foods and they've been doing it for decades. Sure, they're not forcing anyone to do so, but it's plenty effective all the same, all in the name of
  • 1 milligram per milliliter is 1 gram per liter = 0.1% by weight. If I haven't misplaced a decimal, that looks to be roughly the equivalent to 7 or 8 packets of aspartame based artificial sweetener (35mg each) in a glass of water (about 250ml). Pretty sweet. I'm not sure that "only" is the right adverb.

    • by Dastardly ( 4204 )

      https://en.wikipedia.org/wiki/Diet_drink#Amount_of_artificial_sweeteners_in_diet_soft_drinks

      For what it is worth.12 oz can is about 350ml. It looks like 150mg is the top end for artificial sweeteners in a can of a diet drink. So, about 0.5mg/ml or about half what was used in the study.

  • Those early 80s women of fitness drank so much damn Tab and Diet Coke when it first came out. And there has been no great Tab plague even nearly 40 years later. The propaganda on 'fat free' bull shit caused far more harm to western culture health (high calorie, low fat confusion).

    Obviously water is better than heavy sugar drinks or simulated sugar drinks - but freakouts about artificial sweeteners are always more fear than substance. And the real world proves it every day.

  • I wonder if this is part of why people who drink diet soda are just as likely to be obese as people who drink non-diet soda. Aspartame and sucralose are the worst. They give me a screaming headache within minutes. I can tolerate saccharine, though.
    • by TRRosen ( 720617 )

      I'd seek psychiatric help for your imaginary headaches.
      Sorry but aspartame sensitivity is proven to be 100% imaginary. It doesn't exist.

  • From a variety of sources:

    "Sucralose; "a chlorinated derivative of sucrose""

    "Acesulfame K "is not broken down when digested, nor is it stored in the body. After being consumed, it's quickly absorbed by the body and then rapidly excreted, unchanged"

    "After digestion, aspartame is broken down into two amino acids, and methanol."

    "Neotame is recommended to be used in organic food, but it could be causing neurotoxic and immunotoxic damage. These are the same concerns that have been found in aspartame."

    "Laboratory

  • Sugar is bad for you, so avoid it.

    Sugar substitutes are also bad for you. Stop liking what isn't good for you!

    Fuck that. Accept that life is a terminal disease. Look at the various risks and make your own decisions. I choose sugar in moderation.

  • So I guess i should avoid food with 5 times the legal limit of saccharin?

  • Junk science and junkier reporting. But its good to know that if i get an E.coli infection i can just add an entire bottle of sweetener to a diet coke to treat it.

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