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Biotech Medicine

Scientists Determine New Way To Untangle Proteins By Unboiling an Egg 155

An anonymous reader sends word of this biotech breakthrough. "Univ. of California, Irvine (UC Irvine) and Australian chemists have figured out how to unboil egg whites—an innovation that could dramatically reduce costs for cancer treatments, food production and other segments of the $160 billion global biotechnology industry, according to findings published in ChemBioChem. 'Yes, we have invented a way to unboil a hen egg,' said Gregory Weiss, UCI professor of chemistry and molecular biology & biochemistry. 'In our paper, we describe a device for pulling apart tangled proteins and allowing them to refold. We start with egg whites boiled for 20 min at 90 C and return a key protein in the egg to working order.'"
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Scientists Determine New Way To Untangle Proteins By Unboiling an Egg

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  • Wow .... (Score:5, Interesting)

    by gstoddart ( 321705 ) on Monday January 26, 2015 @11:41AM (#48905357) Homepage

    That's pretty cool.

    Imagine, a seasoning which turned your tough steak back from being shoe leather. :-P

    Un-boiling an egg, the mind boggles.

    I wonder what wacky applications chefs will come up with for this one. I can see some of the molecular gastronomy folks doing some odd things.

    • by Anonymous Coward

      Sadly if you RTF[A/S] they say:

      We start with egg whites boiled for 20 min at 90 C and return a key protein in the egg to working order.

      One key protein does not make an egg.

      • Re:Wow .... (Score:5, Funny)

        by FatdogHaiku ( 978357 ) on Monday January 26, 2015 @04:48PM (#48908261)

        Sadly if you RTF[A/S] they say:

        We start with egg whites boiled for 20 min at 90 C and return a key protein in the egg to working order.

        One key protein does not make an egg.

        And we can be thankful for that! Otherwise we'd end up having brunch with some asshat who wants his eggs "unboiled three minutes, served with bearnaise sauce on the side and two slices of sour dough bread toasted to be crisp but not darkened"... and you end up wondering are they going to spit in everyone's food or just his?

      • All jokes aside, maturation of this technique has huge ramifications for the treatment of diseases that are fundamentally due to misfolded proteins. Prion diseases, frightening as they are, might finally have a cure. I would dare say related disorders that involve plaque deposits such as Alzheimer's might also benefit from possible therapeutics.
    • Re:Wow .... (Score:4, Funny)

      by Mr D from 63 ( 3395377 ) on Monday January 26, 2015 @11:55AM (#48905547)

      Un-boiling an egg, the mind boggles.

      I read the article, and still can't figure out what they are really doing or how they are really gonna use it, seems to be a processing technique more than a production technique. Somehow I suspect the unboiled whites are not quite the same as the original.

      I guess the next step is to un-fry a chicken.

      • Re:Wow .... (Score:5, Funny)

        by TWX ( 665546 ) on Monday January 26, 2015 @12:00PM (#48905627)

        I guess the next step is to un-fry a chicken.

        There's a particular Wendy's that I won't eat at; they seem to have mastered this technique based on what I've been served between two pieces of bread.

        • Are you sure that was bread? It might have been more chicken [google.com].
      • Re:Wow .... (Score:5, Informative)

        by Rei ( 128717 ) on Monday January 26, 2015 @01:23PM (#48906547) Homepage

        It's a two-step process. The first is a chemical that dissolves the proteins (still in their "cooked" folding), and the second is some sort of centrifuge or similar (they don't go into details on the device in the article) that subjects the proteins to very high sheer strain, effectively mechanically unfolding them so that they can then relax back into their natural state.

        Not exactly a spice you can sprinkle onto your steak, but still pretty neat. :)

      • Re:Wow .... (Score:4, Informative)

        by Hotawa Hawk-eye ( 976755 ) on Monday January 26, 2015 @01:48PM (#48906807)

        From what I gathered from the article, a particular cancer medication needs to be produced using expensive materials (hamster ovaries) because the proteins produced by the ovaries don't get tangled up for some reason. Producing those proteins in a less expensive material (E. coli, yeast) would lead to tangling of the proteins. If they can use the less expensive material and detangle the proteins for less than the cost of producing the proteins in the hamster ovaries, the price of the medication would (hopefully) go down and the supply would increase.

        So the next step is to un-tangle proteins produced from yeast, I guess.

        • So there's money to be made breeding hamsters for their ovaries?

        • by dargaud ( 518470 )
          As if the price of medications depended on the techniques used to produce them... Ha, you must be new here.
        • by Mortiss ( 812218 )
          Sadly they do not mention any functional tests on the restored protein. Untangling an egg protein and restoration of function by refolding anti-cancer protein (probably enzyme or an antibody) is in a different league since some proteins require specific chaperone accessory proteins to aid in the folding.
      • by Nerrd ( 1094283 )

        I read the article, and still can't figure out what they are really doing or how they are really gonna use it ...

        The article says quite clearly: The scientific problem they're solving has to do with recovering proteins from test tubes in the lab.

        • Thank you for that thorough explanation of the process. The intricate details you presented on how it works are quite well articulated.
      • by gweihir ( 88907 )

        Same here. From the article, I cannot even be sure they can remotely do what the headline implies.

      • Unless you're unfrying four fried chickens, I'm not interested.

    • I can see some of the molecular gastronomy folks doing some odd things.

      Perhaps, but given the possible sources of the acid used, I'm going to avoid eating an egg treated with it.

    • Imagine, a seasoning which turned your tough steak back from being shoe leather.

      I wonder what wacky applications chefs will come up with for this one. I can see some of the molecular gastronomy folks doing some odd things.

      From the article: "he and his colleagues add a urea substance that chews away at the whites, liquefying the solid material." I'm sure if you send that overdone steak back to the kitchen, the chef would be happy to apply the finest urea available to remedy the situation.

      But actually, the process won't return the steak back to uncooked meat, it would turn the steak back to protein soup.

    • It would be an interesting way to eat a "raw" egg without having to worry about salmonella. A new way to make steak tartar, cook all the ingredients, then use new scientific methods to uncook the ingredients. Safe and delicious.
      • It would be an interesting way to eat a "raw" egg without having to worry about salmonella.

        Not really that interesting since pasteurized eggs [safeeggs.com] are already available for sale and relatively easy to find.

      • You can pasteurize eggs and meat at home using any of the precision temperature cookers on the market for Sous Vide cooking. (Immersion circulators, water ovens, crockpot + PID, etc.)
      • Yep, boiling the raw egg in urea would probably turn the Salmonella into harmless bits.

        Your GI track, not so much.....

      • It would be an interesting way to eat a "raw" egg without having to worry about salmonella.

        I eat raw eggs without worrying about salmonella.

        I get my eggs from these guys: http://www.phoenixseggfarm.com... [phoenixseggfarm.com]

        If your egg supply is infected, you should change egg supplier. Those eggs cost more, but they don't make me ill.

        • Re: (Score:3, Informative)

          by Anonymous Coward

          At the bottom of website you posted:

          The FDA now requires that eggs must be packaged and refrigerated within 36 hours. We exceed these newest standards. This minimizes Salmonella from growing in or on the eggs. One should assume, most raw foods may have some level of bacteria and as usual we advise that all eggs be properly cooked to a temperature of 165F to ensure destruction of any Salmonella and to wash hands with soap after handling eggs. After all we do live in a world of microbes.

          Eat safely,

          Phoenix's Egg Farm

          That kinda contradicts your statement.

          • At the bottom of website you posted:

            The FDA now requires that eggs must be packaged and refrigerated within 36 hours. We exceed these newest standards. This minimizes Salmonella from growing in or on the eggs. One should assume, most raw foods may have some level of bacteria and as usual we advise that all eggs be properly cooked to a temperature of 165F to ensure destruction of any Salmonella and to wash hands with soap after handling eggs. After all we do live in a world of microbes.

            Eat safely,

            Phoenix's Egg Farm

            That kinda contradicts your statement.

            No. It suggests a lawyer told them to put that there to cover their arses.
            I've been eating their eggs for some years, because I understand their farming practices are sound, so there is a substantially lower risk of salmonella poisoning than from other supplies.

      • Ever seen century eggs [wikipedia.org]?

      • by jabuzz ( 182671 )

        Or you could just vaccinate your chickens against salmonella (as the U.K. does for example) and stop worrying about it. If you get salmonella from an egg in the U.K. you have eaten something with cheap nasty foreign eggs.

    • I wonder what wacky applications chefs will come up with for this one. I can see some of the molecular gastronomy folks doing some odd things.

      Urea is involved in the protein untangling...that would probably make for some scary gastronomy.

      • Urea is involved in the protein untangling...that would probably make for some scary gastronomy.

        It certainly gives new meaning to "fry until golden". ;-)

    • Re:Wow .... (Score:5, Funny)

      by Marginal Coward ( 3557951 ) on Monday January 26, 2015 @01:14PM (#48906431)

      Un-boiling an egg, the mind boggles.

      Yes, but if these researchers think they're so darn smart, let's see 'em put toothpaste back into the tube...

    • they probably use a strong friggin detergent to untangle everything too. which ingesting might be... you know, terrible for your insides.

  • by MiKom ( 866143 ) on Monday January 26, 2015 @11:42AM (#48905367) Journal
    So much for using egg scrambling as analogue to hash functions.
    • by sinij ( 911942 )
      We will always have car analogies.
    • by TWX ( 665546 )
      boiling != scrambling. Your analogy is safe for now.

      Besides, like brute-force crypto, in some instances it may be possible to un-boil some aspect of the egg, but it appears to be an extremely expensive process carried out by only highly trained professionals and only workable in very specific circumstances, so it's very unlikely that it'll be commonplace in the total number of instances of boiled eggs or encrypted data.
    • by Anonymous Coward

      Yeah, getting stoned from scrambled eggs sounded too good to be true.

      • Yeah, getting stoned from scrambled eggs sounded too good to be true.

        Especially while listening to urea heap.

    • They've unboiled an egg. They haven't unscrambled one.

  • by Mystakaphoros ( 2664209 ) on Monday January 26, 2015 @11:44AM (#48905403) Homepage
    Today I learned that cancer antibodies are often made in hamster ovaries. This may be, to date, the most I have ever thought about hamster ovaries.
    • by Anonymous Coward

      Today I learned that cancer antibodies are often made in hamster ovaries. This may be, to date, the most I have ever thought about hamster ovaries since my last sexual encounter

      FTFY

    • by Anonymous Coward on Monday January 26, 2015 @12:00PM (#48905625)

      This may be, to date, the most I have ever thought about hamster ovaries.

      Your xHamster browsing history says otherwise.

  • by Iniamyen ( 2440798 ) on Monday January 26, 2015 @11:47AM (#48905445)
    This is awesome... but more importantly, can they find a way to un-break a yolk?!
  • by ArcherB ( 796902 ) on Monday January 26, 2015 @11:50AM (#48905485) Journal

    'Yes, we have invented a way to unboil a hen egg,

    Let me know when they can unboil a rooster egg. Now that will be something.

  • by account_deleted ( 4530225 ) on Monday January 26, 2015 @11:56AM (#48905579)
    Comment removed based on user account deletion
    • by itzly ( 3699663 ) on Monday January 26, 2015 @12:14PM (#48905773)

      the flatulence is unbearable (worse than the 2002 methane leak...)

      Methane is odourless. Egg farts stink because of the hydrogen sulfide and similar compounds.

      • Methane itself is odorless. But methane used as fuel (natural gas) has a very smelly sulfur compound added to it so that you will know if there's a leak. Since they said "methane leak" I assume that's what they meant. Your point stands though, a lot of people think methane has a smell because of farts. But farts stink because of hydrogen sulfide and a couple other sulfur compounds.
  • yeah, closed system, whatever.
  • Boiled at 90C? (Score:3, Interesting)

    by magarity ( 164372 ) on Monday January 26, 2015 @12:12PM (#48905749)

    Is their lab at the bottom of death valley or are they using a pressure cooker?
    Every time C vs F comes up, the C fans invariably point to C being vastly superior mainly because 100 C is water's boiling point.

    • by itzly ( 3699663 )

      Higher pressure increases boiling point. And while metric is superior for most things, the Celsius scale is just as arbitrary as Fahrenheit.

    • by raymorris ( 2726007 ) on Monday January 26, 2015 @12:27PM (#48905941) Journal

      Hard-cooked and soft-cooked eggs should be cooked in water just below boiling. Quoting the best selling cookbook in history, Betty Crocker's Cookbook:

      2. Heat to boiling in saucepan; REMOVE FROM HEAT. Cover and let stand 18 minutes. Immediately cool briefly in cold water to prevent further cooking. Tap egg to crack shell; roll egg between hands to loosen shell, then peel.

      (emphasis mine)

      If you keep the water boiling, you get that nasty green film and the albumen becomes rubbery.

    • 100C at sea level. Maybe they're at a higher elevation. Lower pressure = lower boiling point.
    • Is their lab at the bottom of death valley or are they using a pressure cooker?
      Every time C vs F comes up, the C fans invariably point to C being vastly superior mainly because 100 C is water's boiling point.

      "Boiling an egg" really means "heating it in hot water to cause the yolk and albumen to solidify". That can be done at a temperature far below the boiling point of water. This is good because in the summer local news stations can show how hot it is outside because you can "fry an egg on the sidewalk!" complete with a demonstration.

      If I remember correctly 120F is the temperature needed. I used to make a custard ice cream which included a dozen uncooked egg yolks that couldn't be congealed. In order to ac

  • Yes it deals with Hen Egg-White protein, but it's about overcoming electrostatic barriers to crystallization. The first article involves a urea compound, the second a more esoteric complexed metal compound (Tellurium(VI)-Centred Polyoxotungstate). It's not a direct reference to the first link.
  • undead (Score:3, Funny)

    by roman_mir ( 125474 ) on Monday January 26, 2015 @12:21PM (#48905859) Homepage Journal

    So they can restore the denaturated proteins to their original state... which basically is a step towards reviving the dead, however weird that sounds.... Undead chickens will take over.

  • by Anonymous Coward

    Couldn't you just have the chicken eat the egg and just lay a new one?

  • Correct paper link (Score:5, Informative)

    by OverlordQ ( 264228 ) on Monday January 26, 2015 @01:06PM (#48906355) Journal
  • Can it turn your toast back into bread again?
    • Probably not. Your toast, besides having changes in its protein structures, has other physical changes that still can't be undone - we can't convert caramelized sugar back into it's regular form because a portion of that sugar has burned to carbon (part of what forms the color of the toasted bread), the fats burns, as do, actually, some of the proteins.

      But this research does show what could be achieved with judicious use of nanotechnology - perhaps burnt areas could be classified, their carbon atoms mapped,

  • Lysozyme is a very robust protein found in hen egg whites. This process is going to be VERY difficult to apply to other proteins.

    Even with purified lysozyme, you can boil it for extended amounts of time and it will refold on it's own just fine... in very short time scales. You can lyophilize it down to a powder, store it for years, add water... and it will refold and be active. (Lysozyme is an enzyme that degrades bacterial cell walls.)

     

  • Humpty-Dumpty reported to be put back together again.

  • Un-ringing a bell. Stay tuned.

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