Easier Way to Convert Proteins into Crystals 92
Roland Piquepaille writes "As you might know, proteins need to be transformed into 3-D crystals before their atomic structures and their properties can be analyzed. And production of high quality crystals from proteins has been a difficult task until now. But scientists in the U.K. have successfully used a porous medium, or 'nucleant,' a material that encourages protein molecules to crystallize. Their first step towards 'holy grail' of crystallography could help speed up the development of new medicines and treatments."
This is Big (Score:5, Informative)
The reason this bit of news is so big is that it will (hopefully) allow researchers a way to quickly look at the structures of proteins in such as (in the second link) infectious diseases transmitted by prions, or protein particles. Prions seem to be pure protein; they contain neither DNA nor RNA.
If we can understand the shape and formation of proteins, we can understand how viruses and cells work because proteins are the building blocks. Viruses are obviously first on the chopping block as they are the smallest and infect millions of people world wide (AIDS, influenza, the common cold, etc.).
Re:An Even Easier Way to Convert Proteins to Cryst (Score:2, Informative)
Re:This is Big (Score:5, Informative)
The discovery of a 'universal' nucleant (close to the one suggested by the authors of this study) and the development of a matrix to encourage crystallization would greatly speed the screening process, and ultimately, crystallization of proteins.
Understanding protein structure.. (Score:3, Informative)
Re:W00t! (Score:3, Informative)
Roland Piquepaille knows nothing. (Score:1, Informative)
Simply NOT TRUE.
Proteins must be crystalized before they can be analyzed by X-ray crystallography. They can be analyzed by many, many other methods even if they aren't crystals. And frankly, given that proteins aren't in crystalline form in the body, knowing the crystalline form isn't always useful.
NMR (nuclear magnetic resonance) spectroscopy will elucidate the stucture of a protein in solution, which is normally far more useful.
Aside from chemists, biologists & MDs, most people (including paparazzi like Roland)haven't heard of NMR.
Re:This is Big (Score:4, Informative)
Non "obviously" at all. There are countless medical applications for X-ray crystallography. Any time you want to study the structure of a protein it comes in handy. Many diseases are attacked by researchers from the point of view of receptor binding - the binding together of proteins to other compounds like a lock and key. Such receptors act like switches activating or controlling biological processes. These are ubiquitous in nature and understanding the shape of these 'locks' and 'keys' can be useful in trying to understand the mechanisms of all kinds of diseases whether or not they are caused by pathogens.
Re:This is Not so Big (Score:5, Informative)
I'm not a crystallographer, but I work in a lab group that has many crystalographers in it.
It's been known for some time that you can use a variety of materials - including things with porous surfaces, which is what is used here - to assist the process of crystallization. Crystalization is difficult and, frankly, rather unscientific - you take the protein you want to crystallize, and you try different techniques and tricks (of which porous nucleants are an example) until you can get it to work.
So, okay, it would be a "holy grail" if you could find one technique that would let you crystallize most things without going through all that trouble.
However, based on only seven examples (Subscribers only, I'm afraid. [pnas.org]), you absolutely cannot conclude that this is a universal nucleant - based on the similarity among the seven examples, I'd be very surprised if it were; even if it were a universal nucleant, nucleation does not always guarantee usable crystals.
Those caveats aside, it does look like a useful advance.
Re:Ever hear of NMR structure determination? (Score:4, Informative)
Plus, NMR results or more vague than X-Ray crystallography, and can only be used with small proteins, whereas crystallography works for even very large proteins (provided you can get them crystallized).
Re:Ever hear of NMR structure determination? (Score:3, Informative)
I don't know a ton about nuclear medicine. (Score:2, Informative)
The field of nuclear medicine is concerned with things like radiation therapy and PET scanning.
http://science.howstuffworks.com/nuclear-medicine
http://jnm.snmjournals.org/ [snmjournals.org]
http://www.biomedcentral.com/bmcnuclmed/ [biomedcentral.com]
Quibble #2: Your second link is very outdated. Structures for several prion proteins were determined several years ago, using both X-ray diffraction and NMR methods. Science moves on, but many webpages are never updated.
Re:Sounds Like A Lot Of People Here Are Really Sma (Score:2, Informative)