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Skin Cells Turned Embryonic

Posted by ScuttleMonkey on Wed Jun 06, 2007 05:12 PM
from the awaiting-new-objections dept.
anik315 writes "Nature is reporting a major breakthrough in embryonic stem cell research. A straightforward procedure using mouse fibroblasts harvested from the skin can be used to produce pluripotent stem cells that can potentially become any other cell in the body. Not only can Yamanaka's method use the most basic cells, it can be accomplished with simple lab techniques. Possible applications of this breakthrough are to check molecular changes in cells as certain conditions develop. Stem cells produced using this procedure, however, can not be used safely to make genetically matched cells for transplant."
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  • Next step: Embryos (Score:3, Interesting)

    by Anonymous Coward on Wednesday June 06 2007, @05:20PM (#19417491)

    A straightforward procedure using mouse fibroblasts harvested from the skin can be used to produce pluripotent stem cells...

    Just a few more years and it should be possible to cause fibroblasts to grow into embryos. IRC, it's more or less possible now but it involves mixing and matching parts of different cells (the nucleus from the fibroblast and the cytoplasm from a fertilized egg cell.

    Anyway, that should throw the anti-abortion crowd for a loop: "Oh no, he's cut his skin. He's killing babies!" After all, the usual argument is that if something can develop into a human then it should be considered to be a human even before it develops into a human.

    • by Anonymous Coward on Wednesday June 06 2007, @05:23PM (#19417527)

      "Oh no, he's cut his skin. He's killing babies!"

      and yet another demographic will hate emo kids.

    • "the usual argument is that if something can develop into a human then it should be considered to be a human..."

      really? I thought most of the disagreement was over when an embryo/foetus can be considered human.
      (yes there are a few extremists who provide a convenient straw-man)

      I'm pro-choice, btw.
      • I imagine the natural process of conception would be what they are concerned with.

        To date, even in a test tube they are creating life by conception (introducing sperm into an egg for fertilization). If they skipped that part, I think is would be considered synthetic similar to how soy-burgers are supposed to be non meat hamburgers or nylon compared to silk.

        I think the real problem is going to be when the scientist create a conscious life out of skin cells. Or at least attempts to. And there is probably goin
    • "After all, the usual argument is that if something can develop into a human then it should be considered to be a human even before it develops into a human."

      I think the pro-lifers might have something to say about the human-intervention aspect. Namely, that something that could develop into a human being given nine months of waiting is fundamentally different than a cell used as an ingredient in a laboratory process to create embryos.

      The Catholic church, for example, firmly opposes abortion but does not s
      • Re: (Score:3, Insightful)

        Heh, those anti abortion people aren't as stupid as they are portrayed. And this is something that always confused me. Why someone who thinks they are protecting a human life automatically be considered stupid in this one position?
        • by buswolley (591500) on Wednesday June 06 2007, @06:31PM (#19418187) Journal
          Thank you. Indeed, I do see the strengths of pro-abortion supporters, however the pro-life argument is not as weak as it is made out to be.

          Besides, a moderate approach would be to acknowledge that the issue is unclear, or unsolvable, and that it is probably best to error on the side of caution. Even better would be to fund the research of technologies and legislation which can make these issues less relevant.

          For example, let's develop several pre-conception birth control methods which are highly effective. Then require their use in-order to have the privilege of having an abortion. --Like insurance for your car. Responsibility allows the privilege. Plus make this freely available and highly accessible to people of child baring age.

          Advantages:

          1. Reduces unwanted pregnancies.

          2. Reduces abortions.

          3. Re-frames the debate into a more moderate direction, so as to divide our country less.

          4. Makes the whole issue less pressing.

          Thank you for your feedback

          • Re: (Score:3, Funny)

            by Anonymous Coward

            Like insurance for your car.

            heh, more like insurance for the garage.
          • Re: (Score:3, Insightful)

            Besides, a moderate approach would be to acknowledge that the issue is unclear, or unsolvable

            That would be a moderate approach.

            an ... approach would be to acknowledge that the issue is unclear, or unsolvable, and that it is probably best to error on the side of caution.

            This is not a moderate approach. If caution is 'towards killing babies', this is a pro-life approach. If caution is 'towards government invasion of a woman's control of her own body', then it's a pro-choice approach.

            Either way, it's the sam
          • Re: (Score:3, Insightful)

            5. Creates a registry of not only who's having sex, but who is using birth control. No privacy concerns there.

            6. Still requires the Supreme Court to overturn Roe v. Wade.

            Sorry, try again. I, for one, would certainly not consider your proposal moderate. Drastically reducing the amount of freedom women have right now in order to placate a relatively small percentage of the population does not strike me as moderate. "A Modest Proposal" maybe, but definitely not moderate.

            • by sumdumass (711423) on Wednesday June 06 2007, @11:06PM (#19420189) Journal
              There are some deep problems with this proposal. First is that it attempts to vacate the idea that the fetus is an unborn child and acts as if it is a living human intruder. Next, it attempts to treat the body as property in the same way land or a house would be considered property. But it doesn't treat the intent and condition in a relative term. It may be because someone is attempting to justify the action or it maybe because they think a strawman argument rearranged makes more sense.

              To keep it in the same terms, lets put it into perspective to keep the comparison real.

              A woman has the perfect right to evict any intruder from her property, that is, her own body, at any time, for any reason. (Self-ownership is the first principle of liberty.)
              But she doesn't have right to kill the trespasser without giving them enough time to leave. In almost any state, with the exception of Texas maybe, if you find a trespasser/intruder and they pose no direct harm to you or anyone there, you tell them to leave and then kill them because they didn't leave fast enough, you will be going to jail. But pregnancy isn't even a trespasser or intruder, it is a welcomed guest. You have to take certain actions to invite a fetus into your home(body). And in every state, if you invite someone into your house and then kill them, it is murder.

              I know this is someone else's rationalizing. But we can often make judgments to justify something that other see as wacked. You cannot run over a kid playing ball in the street because thats where cars drive when you have plenty of time to stop. Saying the kid shouldn't be playing in the road is just an attempt to justify it to yourself but doesn't make it just. Leaving out the fact that the intruder is crippled and will take a certain amount of time to leave the property and killing them before they can do so because you ordered them out makes no sense either. And repeating this nonsense make even less sense.
              • by Grendel Drago (41496) on Thursday June 07 2007, @08:50AM (#19422829) Homepage

                Don't make the mistake of grouping all pro lifers all into the same group. This is probably the biggest reason your confused about who would support something or why they are supporting it.
                Yeah, but pro-lifers not in the religious group don't really count in a political sense, which means that whatever your nuanced policy measures are which aren't predicated on punishing women for being dirty sluts, nobody's going to bother responding to them.

                Then again, your wailing about "encourag[ing] promiscuity" and how those damned sluts deserve to be punished with unwanted pregnancies because, well, they were asking for it, what with the having sex and all, leads me to believe that your motives may not be that different.
    • by JeanPaulBob (585149) on Wednesday June 06 2007, @05:48PM (#19417743)
      After all, the usual argument is that if something can develop into a human then it should be considered to be a human even before it develops into a human.

      Ah, no.

      The argument is that it can develop into a baby, and that it already is a human.

      I.e., an oak acorn is not a tree, but it is an oak. An blastocyst/embryo is not a baby, but it is a human. A baby is not a toddler, but it is a human. A toddler is not a teenager, but it is a human. A teenager is not an adult, but it is a human (though barely, in come cases ^_^).
    • Re: (Score:3, Interesting)

      Well, no. Stem cells don't develop into babies if they're just out there. Stem cells forming an embryo can turn into a baby. If what you said were true, then amphibians that can regenerate limbs would be able to reproduce asexually, by cuttings. Anyway, that should throw the troll crowd for a loop.
    • After all, the usual argument is that if something can develop into a human then it should be considered to be a human even before it develops into a human.

      Well, no, that's a frequent characterization of the argument by people who themselves believe that an entity does not become human until it emerges from the womb and who fail to understand that not everyone shares that belief, and who therefore create a rationalization for their opponents arguments based on a premise that those opponents reject, and pret

      • Re: (Score:2, Insightful)

        by Anonymous Coward

        The summary seems to suggest that the DNA isn't made well enough even to make perfect transplants for the person donating the skin.

        At present, they use retroviruses to get the four factors that cause the transformation into the cells. The retroviruses mess up the DNA. There may be other methods though besides retroviruses to get the factors into the cells.

        Of course, the factors themselves may also increase the risk that the cells become cancerous - which could turn out to be a harder problem, or not, it's

        • by eli pabst (948845) on Wednesday June 06 2007, @07:22PM (#19418581)
          A person's skin DNA is the same as the rest of their DNA

          Technically it's not. Once a stem cell starts to differentiate, you see different patterns of epigenetic changes that alter which genes are actively expressed and which are silent. It's part of the reason why you don't have eyeball proteins expressed by your feet. In general, we've found that once you start initiating a cascade where a stem cell starts differentiating into something else, it's difficult to go backwards and "undo" the changes.
  • by Maniakes (216039) on Wednesday June 06 2007, @05:28PM (#19417573) Journal
    A straightforward procedure using mouse fibroblasts harvested from the skin can be used to produce pluripotent stem cells [...] Stem cells produced using this procedure, however, can not be use to safely to make genetically matched cells for transplant.

    I think I found the source of the problem.
  • Papers (Score:5, Informative)

    by Anonymous Coward on Wednesday June 06 2007, @05:31PM (#19417607)
    Actual papers for those interested (it was published simultaneously by three groups): (Nature probably requires subscriptions, the first one is free access)

    Nimet Maherali, Rupa Sridharan, Wei Xie, Jochen Utikal, Sarah Eminli, Katrin Arnold, Matthias Stadtfeld, Robin Yachechko, Jason Tchieu, Rudolf Jaenisch, Kathrin Plath, and Konrad Hochedlinger
    http://www.cellstemcell.com/content/article/fullte xt?uid=PIIS1934590907000203 [cellstemcell.com]

    Keisuke Okita, Tomoko Ichisaka & Shinya Yamanaka
    http://www.nature.com/nature/journal/vaop/ncurrent /full/nature05934.html [nature.com]

    Marius Wernig, Alexander Meissner, Ruth Foreman, Tobias Brambrink, Manching Ku, Konrad Hochedlinger, Bradley E. Bernstein & Rudolf Jaenisch
    http://www.nature.com/nature/journal/vaop/ncurrent /full/nature05944.html [nature.com]
  • Who needs high tech when your skin cells can turn you into a bionic person. I just can't wait for the IPO!
  • ... when I hear it's been replicated at a few dozen labs.
  • by Bryan Ischo (893) * on Wednesday June 06 2007, @05:51PM (#19417775) Homepage
    Why are they running these experiments on mouse cells? Why aren't they starting with human skin cells and developing their techniques there? It would avoid the secondary step of having to transfer the technique from mouse tissue to human tissue.

    I always assumed that the reason that experiments are done on mice and other animals is that they are easier to obtain than human subjects and that we can do things to them that would be considered unethical when done to a human (leaving aside some people's feelings that they are unethical when done to animals too).

    But with skin cell experiments, I don't see the reason to do the research on animals. Human skin cells ought to be readily available, ethical to obtain, and ethical to experiment on.

    Why start with mice on this? Why not start with humans and cut one step out of the process?
    • My guess is that these are not just any mice they are using, but "lab grade" mice, whose "properties" are well understood. I am not a biologist, so if someone could help explain this better, please jump in. But basically the mice need to be well characterized so you have some notion of a control when comparing results. That is much more difficult to accomplish with humans.
    • Here's what happens when the ask for volunteers:

      Scientist: Can we 'ave your skin, then?
      Subject: Sod off you plonker!

      Mice don't tend to respond that way. Well they do, but we don't have to listen to mice.

    • by tyler_larson (558763) on Wednesday June 06 2007, @06:52PM (#19418359) Homepage

      Why are they running these experiments on mouse cells? Why aren't they starting with human skin cells and developing their techniques there? It would avoid the secondary step of having to transfer the technique from mouse tissue to human tissue.

      Simplicity. Protocol. Reproducibility.

      Labs that experiment on mice use specific inbred genetic lines that are widely available with limited genetic diversity. This limits the amount of experimental error that can be attributed to the variations in the traits of the animals. It also means that other labs attempting to reproduce the same results will have a greater chance of success because they'll be starting with an organism that genetically is nearly identical to the ones used elsewhere.

      See Model Organism [wikipedia.org].

    • Re: (Score:3, Informative)

      Because then they could be left with an ethical quandry about what to do with any unexpected human embryos that might be produced from the experiments.
  • by WillAffleckUW (858324) on Wednesday June 06 2007, @06:01PM (#19417893) Homepage Journal
    "The four transcription factors used by Yamanaka reprogramme cells inconsistently and inefficiently, so that less than 0.1% of the million cells in a simple skin biopsy will be fully reprogrammed."

    As noted, the major problem is not just the inconsistency, but the locating of the modified cells.

    However, unlike many other slashdot articles, this is is in a peer-reviewed journal, it is based on a technique which has been run for a while and altered based upon other followup work, and it might prove a useful addition for labs to do research, while of limited use in therapeutics.

    But that also depends on cost. People forget that a successful research lab has got to get costs per experiment down - if it costs me $20 per sample and I have a plate of samples, I'll go broke trying to run any sizeable research of any note, especially that with significant data that can answer more than 2 basic questions of statistical significance.
    • Downside: Now we can clone Cowboy Neal by sampling his keyboard.
    • I disagree, dead babies is always the answer.
    • Dammit, and I've been stockpiling dead babies for years waiting for the government to finally start funding embryonic stem cell research. I've got a whole garage full of them, what am I supposed to do now?
    • The poster makes a serious ethical point.

      However emotively he put it, surely it's quite different to take tissue from a consenting human donor than from a subject whose life has just been ended - however "potential" its (his? her?) humanity may be.

      Don't all but the most extreme "it's the woman's body till it's born" zealots regard the abortion of a foetus (with its potential to grow into a human adult) as a necessary evil, rather than a simple lifestyle choice?

      • Re: (Score:3, Insightful)

        >Don't all but the most extreme "it's the woman's body till it's born" zealots regard the abortion of a foetus (with its potential to grow into a human adult) as a necessary evil, rather than a simple lifestyle choice?

        Yup.

        But remember that it's only a fetus after a couple of weeks. Before that, it's an embryo. Bare naming issues aside, you have to draw a line somewhere between a couple of cells and a human being.
        Getting rid of a couple of cells is only a big issue if the morality aspects get blown way ou
      • Re:I knew it.. (Score:4, Insightful)

        by CaptainPatent (1087643) on Wednesday June 06 2007, @05:26PM (#19417555) Journal
        Actually it is. We have already been using skin grafts to cure minor cosmetic flaws from burns or scars with no moral repercussions. I don't see why it would suddenly become immoral to expand that to much more life-threatening diseases and ailments.
            • It's true that a tiny clump of cells with no nervous system doesn't seem much like a person, but I have two young children who not very long ago were very similar tiny clumps of cells. Now, they eat and sleep and play and smile at their Dad, and the older one walks and talks and has all kinds of opinions about things. It seems sad to think that someone would do away with them at an early stage just because they weren't yet able to do all the things they can do now. Just my opinion of course.

              Sure, if you go

      • Re:I knew it.. (Score:5, Insightful)

        by buswolley (591500) on Wednesday June 06 2007, @05:46PM (#19417725) Journal
        Seriously, this is not a scientific question, but a moral/ philosophical question. There IS room for debate. However, as a promising source of embryonic stem cells, this discovery may reduce the importance of the debate. I think that the abortion debate in general should be solved in this way. Make the debate less important by solving the problem of unwanted pregnancies directly with good birth control.
        • Re: (Score:3, Insightful)

          Sure, but the same people who are the biggest advocates against abortion also tend to be the ones that seek to limit access to birth control, so that argument doesn't get very far either.
          • ??? Irrelevant. The advocacy of others does not imply a weakness in my argument.
            • Re: (Score:3, Interesting)

              I meant the argument doesn't get very far in the real world debate because the people you are debating with have these views against birth control as well, not that it is logically flawed in any way.
            • Re: (Score:3, Insightful)

              the most vocal, and therefore the most influential anti-choice proponents claim that birth control for women is tantamount to abortion as most prevent the egg from adhering to the wall of the uterus, or prevent it from traveling down the fallopian tubes, where it can grow further. Condoms, therefore, can be the only one they can support, but those same supporters tend to be the ones who want to preach abstinence because their head seems firmly implanted in the sand, doomed to pretend people from kids to gra
          • the same people who are the biggest advocates against abortion also tend to be the ones that seek to limit access to birth control, so that argument doesn't get very far either.

            Sure it does, if reasonable people can ignore the others. The problem is unwanted pregnancy and reasonable people can work together to reduce it and support the people who have the problem. The use of obnoxious and confused advocates is an underhanded way to kill off a proposal.

            The counterexamples are communists, extreme femi

            • Re: (Score:3, Informative)

              You don't have to be religious or hate sex to think that abortion is murder. In almost all cases, if no one does anything to a pregnant woman, a child will be born.

              Actually, the fetus will naturally miscarry a significant percentage of the time, with that percentage getting fairly high depending on various factors. Is a 45 year old women who gets pregnant guilty of reckless endangerment?

              http://en.wikipedia.org/wiki/Miscarriage#Prevalenc e [wikipedia.org]

              Prospective studies using very sensitive early pregnancy tests have f

        • Re: (Score:3, Insightful)

          Why is the abortion debate involved in this at all? Stem cells used in research are not acquired from abortions! Abortions are a terrible source of stem cells for research purposes.
    • Re: (Score:3, Insightful)

      <blockquote>what is the justification for using public money for research that tens of millions of people consider murder</blockquote>
      Frankly, I think we should do it just out of spite... for people who would spout the kind of self-important ignorant garbage that just evacuated itself from the barren environment of your skull.
    • by EMeta (860558) on Wednesday June 06 2007, @08:47PM (#19419275)
      Without mod points, I must resort to replying to a highly ranked troll. Sigh.

      First, if you would RTFS, much less article, much less paper, you would know that one of the fascinating things about this procedure is that it uses skin cells, not embryonic cells as a base. Very few people who believe in any invasive medicine have a problem with this. This is a breakthrough in part because it fixes problems like embryonic harvesting or even The Island-esqe people harvesting because a given sick person could use it on him or herself.

      Secondly, a work force that lives productively into their 80's would be a lovely thing for any society's economies. A government should certainly be concerned about its nation's economy, yes?

      Thirdly, medical research=good for people. Democratic government=group that uses pooled funds for betterness of group. Are there spending issues? Duh. But still better than most systems. I want to put my money in a pool that can fund science. Hooray that there is an automatic way that this happens for me. I don't even need said science to produce economic results for me to be happy about it. But if it's going to, I won't turn that down.

      And for the record, a considerable majority of Americans do want stem cell research, even from embryos. Google news reports around last Nov's Missouri senate elections, & there were several stories about how while most Americans support it, it's a non-issue in the polls.

    • Re: (Score:3, Interesting)

      To answer your queries

      1) It's worthwhile pursuing other avenues of research in case this avenue doesn't pan out

      2) It's worthwhile pursuing other avenues of research that use stem cells from normal sources until this method is more reliable in producing the raw materials for those complementary avenues of research. Those other avenues of research also add to our understanding of cell differentiation which might provide positive feedback into your favoured avenues of research.

      3) The pharmaceutical industry is
    • by tfoss (203340) on Wednesday June 06 2007, @10:23PM (#19419875)
      I can't believe this hogwash was moderated 'insightful.' Ignorant pseudo-libertarian ranting apparently fulfills some slashdot community need.

      With so many unquestionably moral methods of creating stem cells on the very near horizon


      We hope this is so, but have absolutely no way of knowing.

      what is the justification for using public money for research that tens of millions of people consider murder?


      First off, I'm not sure I buy your proclamation (where are the tens of millions fighting against in-vitro fertilization). Tens of millions also consider eating animals tantamount to murder...let's kill off the USDA. Tens of millions believe in creationism, let's stop geology/archeology/cosmology research. Tens of millions of people believe lots of crazy shit that should not be directing gov't policy, thats the way democracy works.

      Additionally, no stem-cell research that I know of is focused on any public health concern such as communicable diseases; rather, it is focused on private health issues such as cancer or Parkinson's disease. Hence, it is debatable whether such research is the domain of government at all.

      1. *That* you know of. Even the researchers don't really know how widely stem-cell therapy might or might not be used, that's why you research it. 2. So you are positing that only those lucky enough to have suffered from a communicable disease should be a concern of the gov't? Really? And what defines a public health concern? Cancer from industrial pollution? vCJD from mad-cows...that happens to be similar to parkinson's (and alzheimer's)? So people who had the unfortunate luck to be born with a disease are SOL, yet those with preventable sexually-transmitted diseases are the beneficiaries? What the hell kind of moral system did you pluck that from?

      If the government is going to intrude so deeply into the private sphere, should it not do so under only the most benign of manners?

      Right, so how about, say, abortion? Or euthanasia? Should the gov't butt out of those 'private spheres'? And if so, you've lost the support of those "tens of millions" who have an issue with stem-cell research.

      In contrast, there is no compelling reason for the government to fund stem-cell research at all...and even less so, given its controversy.

      Well, public support for gov't funding of research is a pretty damn compelling reason. Again, democracy and all. If you can convince a majority to do away with basic research funding, then we can have a debate about the societal benefit of gov't support of research. Until then, we, as a society, have pretty clearly decided that it is in our interest to support research (of non-communicable diseases as well as stem-cell related technology).


      -Ted